Reporter

Part:BBa_K3871005

Designed by: Yoav Navott   Group: iGEM21_TAU_Israel   (2021-10-21)

Description

mCherry is a red fluorescent protein used as a reporter derived from the fluorophore DsRed, which was originally isolated from Discosoma sea anemones. It is commonly used due to its colour and photostability compared to other monomeric fluorophores.

This part is a variant of mCherry, the sequence of which was modified to optimize its expression in Bacillus subtilis against E. coli using the Communique tool developed by the 2021 TAU Israel team. This specific variant was optimized based on typical decoding rate ratio between B. subtilis and E. coli. You may read more about the optimization model here.


For more information on this part, please consult the 2021 TAU Israel wiki results page found here. Information about experimental procedures can be found here and here.


mCherry, TDR-R optimized for Bacillus subtilis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 364
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 364
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 364
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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